Hotspot

rps2

LSC · 16385-17068 · 684 bp

Back to Dipteronia

Pi 0.142

MarkerSeek score 82.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 94
pi: 0.142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	AAAAGAGCACCGGACCAATT	GTTTTCAAGAAACCGCTCGG	45.0	50.0	58.0	58.0	1759-1770 bp	1.0	88.5
rps2_p2	AAAAGAGCACCGGACCAATT	CGCTCGGGTTTTAGCAAAAG	45.0	50.0	58.0	58.0	1746-1757 bp	1.0	88.5
rps2_p3	AAAAGAGCACCGGACCAATT	TTGGATTGTTACGAGCGGAG	45.0	50.0	58.0	58.0	1879-1890 bp	1.0	88.2
rps2_p4	AAAAGAGCACCGGACCAATT	TTACCCGGCGAACTTATTGG	45.0	50.0	58.0	58.0	1895-1906 bp	1.0	88.0
rps2_p5	ACCGGACCAATTCGAAACAA	TTGGATTGTTACGAGCGGAG	45.0	50.0	58.0	58.0	1871-1882 bp	1.0	88.0