Hotspot

rpl32-trnL-UAG

SSC · 115434-116530 · 1097 bp

Back to Dipteronia

Pi 0.086
MarkerSeek score 88.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1097
variable_sites
89
pi
0.086
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCTTTCGACCGGGAATTCAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1151-1184 bp 1.0 88.6
rpl32-trnL-UAG_p2 CCTTTCGACCGGGAATTCAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1152-1185 bp 1.0 88.5
rpl32-trnL-UAG_p3 CCTTTCGACCGGGAATTCAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1161-1194 bp 1.0 87.6
rpl32-trnL-UAG_p4 CCTTTCGACCGGGAATTCAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.2 57.8 1160-1193 bp 1.0 87.0
rpl32-trnL-UAG_p5 CCTTTCGACCGGGAATTCAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.2 57.8 1159-1192 bp 1.0 87.0