Hotspot

trnW-CCA-trnP-UGG

LSC · 68305-68511 · 207 bp

Back to Dipteronia

Pi 0.0495
MarkerSeek score 73.90
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
207
variable_sites
10
pi
0.0495
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 269-274 bp 1.0 83.3
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 271-276 bp 1.0 83.2
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 272-277 bp 1.0 83.2
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 282-287 bp 1.0 83.1
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 283-288 bp 1.0 83.1