Hotspot

trnG-UCC-trnfM-CAU

LSC · 37967-38131 · 165 bp

Back to Dipteronia

Pi 0.0446

MarkerSeek score 73.20

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 7
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	271-279 bp	1.0	82.8
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	256-264 bp	1.0	81.5
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	255-263 bp	1.0	81.5
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	AGGTCATGGGTTCAAATCCC	50.0	50.0	57.8	57.5	231-239 bp	1.0	79.5
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CCTTGAGGTCATGGGTTCAA	50.0	50.0	57.8	57.4	236-244 bp	1.0	79.0