Hotspot

atpH-atpI

LSC · 14273-15420 · 1148 bp

Back to Dipteronia

Pi 0.0252

MarkerSeek score 76.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 28
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCGGCG	45.0	45.0	58.1	58.5	1198-1234 bp	1.0	79.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCGGCG	50.0	45.0	57.9	58.5	1256-1292 bp	1.0	79.0
atpH-atpI_p3	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCGGCG	55.0	45.0	58.2	58.5	1260-1296 bp	1.0	78.4
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TAGGCGAATCTATGGAGGGC	45.0	55.0	58.1	58.7	1171-1207 bp	1.0	77.7
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TAGGCGAATCTATGGAGGGC	50.0	55.0	57.9	58.7	1229-1265 bp	1.0	77.4