Hotspot

trnR-UCU-atpA

LSC · 10800-11085 · 286 bp

Back to Dipteronia

Pi 0.0927

MarkerSeek score 77.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 23
pi: 0.0927
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TCAGGAACAAATGGAACGCT	47.6	45.0	57.3	57.7	313-351 bp	1.0	73.8
trnR-UCU-atpA_p2	TGGATAGGACAGAGGTCTTCT	TCAGGAACAAATGGAACGCT	47.6	45.0	56.9	57.7	348-386 bp	1.0	70.4
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	TCAGGAACAAATGGAACGCT	42.9	45.0	56.6	57.7	312-350 bp	1.0	68.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TGGAACGCTTTCTACTTCAGG	47.6	47.6	57.3	58.0	302-340 bp	1.0	67.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TTCAGGAACAAATGGAACGC	47.6	45.0	57.3	56.9	314-352 bp	1.0	67.4