Hotspot

trnS-GCU-trnG

LSC · 8973-9766 · 794 bp

Back to Dipteronia

Pi 0.104

MarkerSeek score 89.30

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 77
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	859-882 bp	1.0	81.1
trnS-GCU-trnG_p2	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	859-882 bp	1.0	81.1
trnS-GCU-trnG_p3	GCTCGTACAACGGATTAGCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.1	58.2	881-904 bp	1.0	80.3
trnS-GCU-trnG_p4	GCTCGTACAACGGATTAGCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.1	56.8	881-904 bp	1.0	80.3
trnS-GCU-trnG_p5	AGCTCGTACAACGGATTAGC	ACGAATCGCACTTTTACCACT	50.0	42.9	57.8	58.2	882-905 bp	1.0	79.4