Hotspot

trnK-UUU-rps16

LSC · 4472-5495 · 1024 bp

Back to Dipteronia

Pi 0.0499
MarkerSeek score 76.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1024
variable_sites
49
pi
0.0499
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAGGGATCCTGGAACAAGGA 55.0 50.0 58.0 57.9 1653-1679 bp 1.0 84.2
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AGGGATCCTGGAACAAGGAA 55.0 50.0 58.0 57.9 1652-1678 bp 1.0 84.2
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG CGACAGGAACGGTTCATGAT 55.0 50.0 58.0 58.0 1218 bp 0.5 59.8
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT CGACAGGAACGGTTCATGAT 50.0 50.0 58.1 58.0 1214 bp 0.5 59.3
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT CGACAGGAACGGTTCATGAT 50.0 50.0 58.1 58.0 1215 bp 0.5 59.3