Hotspot

ycf1

SSC · 102036-107582 · 5547 bp

Back to Dipterocarpus

Pi 0.00607

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 89
pi: 0.00607
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATCTATTATGCGCCGCT	CCAAAAGTTGCCAAACTGGG	45.0	50.0	57.5	58.0	1097-1208 bp	1.0	81.0
ycf1_p2	GCGTTCACGGTTTATTCCTG	CCAAAAGTTGCCAAACTGGG	50.0	50.0	57.5	58.0	943-1054 bp	1.0	80.8
ycf1_p3	CAATCTATTATGCGCCGCTG	CCAAAAGTTGCCAAACTGGG	50.0	50.0	57.5	58.0	1096-1207 bp	1.0	80.6
ycf1_p4	TCAATCTATTATGCGCCGCT	TGCCAAACTGGGAAGGTATG	45.0	50.0	57.5	57.8	1089-1200 bp	1.0	79.7
ycf1_p5	TCAATCTATTATGCGCCGCT	GCCAAACTGGGAAGGTATGT	45.0	50.0	57.5	57.8	1088-1199 bp	1.0	79.7