Hotspot

petB

LSC · 69379-70836 · 1458 bp

Back to Dipterocarpus

Pi 0.00167

MarkerSeek score 55.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 6
pi: 0.00167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GCCCAGTCTGTTTTGGTAGT	ACTATTGGCTGTTCCTCCCC	50.0	55.0	57.7	59.1	1569-1589 bp	1.0	70.5
petB_p2	GCCCAGTCTGTTTTGGTAGT	GCTGTTCCTCCCCCAAGTAA	50.0	55.0	57.7	59.3	1562-1582 bp	1.0	68.7
petB_p3	AGCCCAGTCTGTTTTGGTAG	ACTATTGGCTGTTCCTCCCC	50.0	55.0	57.4	59.1	1570-1590 bp	1.0	68.1
petB_p4	TGTTTTGGTAGTTCGACCGT	ACTATTGGCTGTTCCTCCCC	45.0	55.0	57.4	59.1	1561-1581 bp	1.0	67.9
petB_p5	GCCCAGTCTGTTTTGGTAGT	CTGTTCCTCCCCCAAGTAAT	50.0	50.0	57.7	56.5	1561-1581 bp	1.0	67.1