Hotspot

clpP

LSC · 65087-66503 · 1417 bp

Back to Dipterocarpus

Pi 0.0153

MarkerSeek score 49.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1417
variable_sites: 50
pi: 0.0153
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACCAACGGAAACCATAA	TGTTACGCTTCCACGTCAAA	45.0	45.0	58.0	58.1	1936-1952 bp	1.0	83.5
clpP_p2	GATTGGCTGTCTTGCGTTTC	TCCAAAAACCCGGATTCGTT	50.0	45.0	58.0	58.0	2346-2456 bp	1.0	83.3
clpP_p3	TGCACCAACGGAAACCATAA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2576-2686 bp	1.0	83.2
clpP_p4	TTGCACCAACGGAAACCATA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2577-2687 bp	1.0	83.2
clpP_p5	TGCACCAACGGAAACCATAA	TTGTTACGCTTCCACGTCAA	45.0	45.0	58.0	58.1	1940-1953 bp	0.666667	68.8