Hotspot

accD

LSC · 51554-53188 · 1635 bp

Back to Dipterocarpus

Pi 0.0161

MarkerSeek score 65.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1635
variable_sites: 66
pi: 0.0161
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAGAGGAAAGGGGGAAACT	TCGAATTTGCTTTCCTGGGA	50.0	45.0	57.9	57.4	1691-1814 bp	1.0	81.4
accD_p2	TCAGAGGAAAGGGGGAAACT	TCGAATTTGCTTTCCTGGGAA	50.0	42.9	57.9	58.1	1691-1814 bp	1.0	77.0
accD_p3	TCAGAGGAAAGGGGGAAACT	TTCGAATTTGCTTTCCTGGGA	50.0	42.9	57.9	58.1	1692-1815 bp	1.0	77.0
accD_p4	TCAGAGGAAAGGGGGAAACT	CGAATTTGCTTTCCTGGGAAG	50.0	47.6	57.9	57.8	1690-1813 bp	1.0	76.2
accD_p5	TCAGAGGAAAGGGGGAAACT	CGAATTTGCTTTCCTGGGAA	50.0	45.0	57.9	56.6	1690-1813 bp	1.0	74.8