Hotspot

trnL-UAG-rpl32

SSC · 117400-118761 · 1362 bp

Back to Dipterocarpus

Pi 0.00503

MarkerSeek score 56.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1362
variable_sites: 16
pi: 0.00503
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AAAAGGCCCGAAGTAGCAAA	AAGTGGACTGCGACAACAAT	45.0	45.0	58.0	58.0	2383-2444 bp	1.0	83.2
trnL-UAG-rpl32_p2	CAGCGTGTCTACCAATTCCA	AAGTGGACTGCGACAACAAT	50.0	45.0	57.9	58.0	1861-1902 bp	1.0	83.1
trnL-UAG-rpl32_p3	AGCGTGTCTACCAATTCCAC	AAGTGGACTGCGACAACAAT	50.0	45.0	57.9	58.0	1860-1901 bp	1.0	83.1
trnL-UAG-rpl32_p4	ACGACTGAAACGACCGAAAA	AAGTGGACTGCGACAACAAT	45.0	45.0	58.1	58.0	2092-2153 bp	1.0	83.0
trnL-UAG-rpl32_p5	AAAAGGCCCGAAGTAGCAAA	CGGTAGATAAGTGGACTGCG	45.0	55.0	58.0	57.9	2391-2452 bp	1.0	82.5