Hotspot

rps12-clpP

LSC · 64881-65086 · 206 bp

Back to Dipterocarpus

Pi 0.0395

MarkerSeek score 65.00

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 206
variable_sites: 17
pi: 0.0395
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGCACCAACGGAAACCATAA	AACGTATGGCATTCCCTCAC	45.0	50.0	58.0	58.0	721-735 bp	1.0	85.0
rps12-clpP_p2	TTGCACCAACGGAAACCATA	AACGTATGGCATTCCCTCAC	45.0	50.0	58.0	58.0	722-736 bp	1.0	85.0
rps12-clpP_p3	TGCACCAACGGAAACCATAA	CTGGGTGATAGCCTGGGATA	45.0	55.0	58.0	58.0	572 bp	0.166667	46.3
rps12-clpP_p4	TTGCACCAACGGAAACCATA	CTGGGTGATAGCCTGGGATA	45.0	55.0	58.0	58.0	573 bp	0.166667	46.3
rps12-clpP_p5	GATTGGCTGTCTTGCGTTTC	CTGGGTGATAGCCTGGGATA	50.0	55.0	58.0	58.0	342 bp	0.166667	46.2