Hotspot

rpl33-rps18

LSC · 62434-63067 · 634 bp

Back to Dipterocarpus

Pi 0.0331

MarkerSeek score 78.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 51
pi: 0.0331
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAT	45.0	45.0	56.2	57.1	657-723 bp	1.0	68.7
rpl33-rps18_p2	AACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAT	42.9	45.0	57.0	57.1	658-724 bp	1.0	66.8
rpl33-rps18_p3	ACATACAATTCACGGGGAGA	GGCAAACGCCTACGAAAAGA	45.0	50.0	56.2	59.1	658-724 bp	1.0	66.8
rpl33-rps18_p4	ACATACAATTCACGGGGAGA	AATTTGATCCCCCGATTGGA	45.0	45.0	56.2	56.9	683-749 bp	1.0	66.5
rpl33-rps18_p5	ACATACAATTCACGGGGAGA	CAATTTGATCCCCCGATTGG	45.0	50.0	56.2	56.8	684-750 bp	1.0	66.1