Hotspot

psaJ-rpl33

LSC · 61745-62232 · 488 bp

Back to Dipterocarpus

Pi 0.0082
MarkerSeek score 56.90
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
488
variable_sites
11
pi
0.0082
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CGTAGCACCGGTACTAAGTG TCTGGAAATGCCCGTTGATT 55.0 45.0 57.8 57.8 693-713 bp 1.0 78.3
psaJ-rpl33_p2 CGTAGCACCGGTACTAAGTG ATCTGGAAATGCCCGTTGAT 55.0 45.0 57.8 57.6 694-714 bp 1.0 76.5
psaJ-rpl33_p3 CCAGATGCGTTGACATTTCC TCTGGAAATGCCCGTTGATT 50.0 45.0 57.5 57.8 620-640 bp 1.0 75.5
psaJ-rpl33_p4 CCAGATGCGTTGACATTTCC ATCTGGAAATGCCCGTTGAT 50.0 45.0 57.5 57.6 621-641 bp 1.0 73.7
psaJ-rpl33_p5 CCGTAGCACCGGTACTAAGT TCTGGAAATGCCCGTTGATT 55.0 45.0 58.9 57.8 694-714 bp 1.0 72.6