Hotspot

psbE-petL

LSC · 59175-60293 · 1119 bp

Back to Dipterocarpus

Pi 0.00268

MarkerSeek score 60.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 9
pi: 0.00268
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTACTCAGACCGA	50.0	45.5	57.9	57.9	1232-1325 bp	1.0	67.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTACTCAGACCGA	50.0	45.5	57.8	57.9	1155-1248 bp	1.0	66.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTCGTATCTTACTCAGACCGA	45.0	45.5	58.2	57.9	1241-1334 bp	1.0	66.1
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGTCGTATCTTACTCAGACCGA	50.0	45.5	58.2	57.9	1246-1339 bp	1.0	66.0
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGTCGTATCTTACTCAGACCGA	50.0	45.5	57.8	57.9	1240-1333 bp	1.0	66.0