Hotspot

petA-psbJ

LSC · 57448-58381 · 934 bp

Back to Dipterocarpus

Pi 0.011

MarkerSeek score 70.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 24
pi: 0.011
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTTTACAAGGCCTTTTCCT	TATTCTGGGTTGGGTTCGTC	42.9	50.0	58.1	57.2	1055-1092 bp	1.0	69.7
petA-psbJ_p2	ACGTTTACAAGGCCTTTTCCT	CATATTCTGGGTTGGGTTCGT	42.9	47.6	58.1	58.0	1057-1094 bp	1.0	67.9
petA-psbJ_p3	ACAAGGCCTTTTCCTCTTCT	TATTCTGGGTTGGGTTCGTC	45.0	50.0	56.6	57.2	1049-1086 bp	1.0	67.4
petA-psbJ_p4	ACGTTTACAAGGCCTTTTCC	TATTCTGGGTTGGGTTCGTC	45.0	50.0	56.5	57.2	1055-1092 bp	1.0	66.5
petA-psbJ_p5	ACGTTTACAAGGCCTTTTCCT	ACGTTAGCTGGTATTCCTGTG	42.9	47.6	58.1	57.8	1109-1146 bp	1.0	65.7