Hotspot

trnD-GUC-trnY-GUA

LSC · 22455-22908 · 454 bp

Back to Dipterocarpus

Pi 0.0108
MarkerSeek score 64.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
454
variable_sites
10
pi
0.0108
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CTTGGGGATGAATTGCTGGT AATTGATTCGTCCTGGGTCG 50.0 50.0 58.1 58.0 665-683 bp 1.0 80.2
trnD-GUC-trnY-GUA_p2 TCCTTGGGGATGAATTGCTG AATTGATTCGTCCTGGGTCG 50.0 50.0 57.9 58.0 667-685 bp 1.0 80.2
trnD-GUC-trnY-GUA_p3 TGTGTTATTGGATCCGTCGG AATTGATTCGTCCTGGGTCG 50.0 50.0 57.7 58.0 642-660 bp 1.0 79.0
trnD-GUC-trnY-GUA_p4 TGGGGATGAATTGCTGGTTT AATTGATTCGTCCTGGGTCG 45.0 50.0 57.7 58.0 663-681 bp 1.0 78.8
trnD-GUC-trnY-GUA_p5 TTGGGGATGAATTGCTGGTT AATTGATTCGTCCTGGGTCG 45.0 50.0 57.7 58.0 664-682 bp 1.0 78.8