primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CTTGGGGATGAATTGCTGGT	AATTGATTCGTCCTGGGTCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.167547	22455	22908	665-683	1.000000	80.2
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TCCTTGGGGATGAATTGCTG	AATTGATTCGTCCTGGGTCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.172621	22455	22908	667-685	1.000000	80.2
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	TGTGTTATTGGATCCGTCGG	AATTGATTCGTCCTGGGTCG	20	20	50.0	50.0	57.7	58.0	4.4	0.0	0.327339	22455	22908	642-660	1.000000	79.0
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	TGGGGATGAATTGCTGGTTT	AATTGATTCGTCCTGGGTCG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.348174	22455	22908	663-681	1.000000	78.8
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TTGGGGATGAATTGCTGGTT	AATTGATTCGTCCTGGGTCG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.348174	22455	22908	664-682	1.000000	78.8
trnE-UUC-psbD_p1	trnE-UUC-psbD	1	CGTAGTATCCTACCCCCAGG	GAAACGGTCCCTACGTAACC	20	20	60.0	55.0	58.1	58.0	0.0	5.0	0.083098	23125	25655	2593-2698	1.000000	84.0
trnE-UUC-psbD_p2	trnE-UUC-psbD	2	GCTGCCTCCTTGAAAGAGAG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.3	58.0	0.0	5.0	0.267097	23125	25655	2558-2663	1.000000	82.6
trnE-UUC-psbD_p3	trnE-UUC-psbD	3	TGTCCTGAACCACTAGACGA	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	57.7	58.0	0.0	5.0	0.285247	23125	25655	2537-2642	1.000000	82.5
trnE-UUC-psbD_p4	trnE-UUC-psbD	4	CGTAGTATCCTACCCCCAGG	ACAAAAACGAAACGGTCCCT	20	20	60.0	45.0	58.1	58.2	0.0	0.0	0.325459	23125	25655	2601-2706	1.000000	82.0
trnE-UUC-psbD_p5	trnE-UUC-psbD	5	CGTAGTATCCTACCCCCAGG	GACCGGACCAACCTACAAAA	20	20	60.0	50.0	58.1	57.7	0.0	9.5	0.338656	23125	25655	2615-2720	1.000000	81.9
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	57.9	57.8	0.0	1.1	0.289634	28955	29685	856-889	1.000000	82.9
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.9	57.8	0.0	1.1	0.304034	28955	29685	869-902	1.000000	82.7
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	28955	29685	859-892	1.000000	82.2
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.428804	28955	29685	855-888	1.000000	81.8
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	20	20	55.0	50.0	57.9	58.3	0.0	7.1	0.433447	28955	29685	895-928	1.000000	81.7
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGTTAGTCCCGGTAAAGCCC	AGTATTTGAGCCCGCTTAGC	20	20	55.0	50.0	59.1	58.0	0.0	0.0	1.137587	39629	40213	692-744	1.000000	78.1
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AGTTAGTCCCGGTAAAGCCC	GTATTTGAGCCCGCTTAGCT	20	20	55.0	50.0	59.1	58.0	0.0	1.8	1.137587	39629	40213	691-743	1.000000	78.1
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CCCCAGACGGCGTATTTTT	AGTATTTGAGCCCGCTTAGC	19	20	52.6	50.0	58.1	58.0	14.7	0.0	1.182335	39629	40213	674-726	1.000000	77.8
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CCCCAGACGGCGTATTTTT	GTATTTGAGCCCGCTTAGCT	19	20	52.6	50.0	58.1	58.0	14.7	1.8	1.182335	39629	40213	673-725	1.000000	77.8
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	59.1	57.9	0.0	0.0	1.225254	39629	40213	673-725	1.000000	77.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	40287	41271	1035-1058	1.000000	80.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	40287	41271	1043-1066	1.000000	80.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAGAAATAGGGAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	21.9	0.0	0.240656	40287	41271	1091-1114	1.000000	79.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	40287	41271	1067-1090	1.000000	79.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAGAAATAGGGAAAGCCGGC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.2	58.1	21.9	0.0	0.305465	40287	41271	1099-1122	1.000000	79.2
accD_p1	accD	1	TCAGAGGAAAGGGGGAAACT	TCGAATTTGCTTTCCTGGGA	20	20	50.0	45.0	57.9	57.4	0.0	0.0	0.711464	51554	53188	1691-1814	1.000000	81.4
accD_p2	accD	2	TCAGAGGAAAGGGGGAAACT	TCGAATTTGCTTTCCTGGGAA	20	21	50.0	42.9	57.9	58.1	0.0	0.0	1.263683	51554	53188	1691-1814	1.000000	77.0
accD_p3	accD	3	TCAGAGGAAAGGGGGAAACT	TTCGAATTTGCTTTCCTGGGA	20	21	50.0	42.9	57.9	58.1	0.0	0.0	1.263683	51554	53188	1692-1815	1.000000	77.0
accD_p4	accD	4	TCAGAGGAAAGGGGGAAACT	CGAATTTGCTTTCCTGGGAAG	20	21	50.0	47.6	57.9	57.8	0.0	10.6	1.355822	51554	53188	1690-1813	1.000000	76.2
accD_p5	accD	5	TCAGAGGAAAGGGGGAAACT	CGAATTTGCTTTCCTGGGAA	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.528241	51554	53188	1690-1813	1.000000	74.8
petA-psbJ_p1	petA-psbJ	1	ACGTTTACAAGGCCTTTTCCT	TATTCTGGGTTGGGTTCGTC	21	20	42.9	50.0	58.1	57.2	17.6	0.0	1.833834	57448	58381	1055-1092	1.000000	69.7
petA-psbJ_p2	petA-psbJ	2	ACGTTTACAAGGCCTTTTCCT	CATATTCTGGGTTGGGTTCGT	21	21	42.9	47.6	58.1	58.0	17.6	0.0	2.060510	57448	58381	1057-1094	1.000000	67.9
petA-psbJ_p3	petA-psbJ	3	ACAAGGCCTTTTCCTCTTCT	TATTCTGGGTTGGGTTCGTC	20	20	45.0	50.0	56.6	57.2	17.6	0.0	2.131730	57448	58381	1049-1086	1.000000	67.4
petA-psbJ_p4	petA-psbJ	4	ACGTTTACAAGGCCTTTTCC	TATTCTGGGTTGGGTTCGTC	20	20	45.0	50.0	56.5	57.2	17.6	0.0	2.232037	57448	58381	1055-1092	1.000000	66.5
petA-psbJ_p5	petA-psbJ	5	ACGTTTACAAGGCCTTTTCCT	ACGTTAGCTGGTATTCCTGTG	21	21	42.9	47.6	58.1	57.8	17.6	0.0	2.306847	57448	58381	1109-1146	1.000000	65.7
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTACTCAGACCGA	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.185713	59175	60293	1232-1325	1.000000	67.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTACTCAGACCGA	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.290722	59175	60293	1155-1248	1.000000	66.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGTCGTATCTTACTCAGACCGA	20	22	45.0	45.5	58.2	57.9	0.0	0.0	2.305400	59175	60293	1241-1334	1.000000	66.1
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AGTCGTATCTTACTCAGACCGA	20	22	50.0	45.5	58.2	57.9	0.0	0.0	2.318305	59175	60293	1246-1339	1.000000	66.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGTCGTATCTTACTCAGACCGA	20	22	50.0	45.5	57.8	57.9	0.0	0.0	2.321730	59175	60293	1240-1333	1.000000	66.0
psaJ-rpl33_p1	psaJ-rpl33	1	CGTAGCACCGGTACTAAGTG	TCTGGAAATGCCCGTTGATT	20	20	55.0	45.0	57.8	57.8	16.3	0.0	0.398761	61745	62232	693-713	1.000000	78.3
psaJ-rpl33_p2	psaJ-rpl33	2	CGTAGCACCGGTACTAAGTG	ATCTGGAAATGCCCGTTGAT	20	20	55.0	45.0	57.8	57.6	16.3	0.0	0.626621	61745	62232	694-714	1.000000	76.5
psaJ-rpl33_p3	psaJ-rpl33	3	CCAGATGCGTTGACATTTCC	TCTGGAAATGCCCGTTGATT	20	20	50.0	45.0	57.5	57.8	0.0	0.0	0.764009	61745	62232	620-640	1.000000	75.5
psaJ-rpl33_p4	psaJ-rpl33	4	CCAGATGCGTTGACATTTCC	ATCTGGAAATGCCCGTTGAT	20	20	50.0	45.0	57.5	57.6	0.0	0.0	0.991870	61745	62232	621-641	1.000000	73.7
psaJ-rpl33_p5	psaJ-rpl33	5	CCGTAGCACCGGTACTAAGT	TCTGGAAATGCCCGTTGATT	20	20	55.0	45.0	58.9	57.8	21.6	0.0	1.116326	61745	62232	694-714	1.000000	72.6
rpl33-rps18_p1	rpl33-rps18	1	ACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAT	20	20	45.0	45.0	56.2	57.1	0.0	0.0	2.654895	62434	63067	657-723	1.000000	68.7
rpl33-rps18_p2	rpl33-rps18	2	AACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAT	21	20	42.9	45.0	57.0	57.1	0.0	0.0	2.887250	62434	63067	658-724	1.000000	66.8
rpl33-rps18_p3	rpl33-rps18	3	ACATACAATTCACGGGGAGA	GGCAAACGCCTACGAAAAGA	20	20	45.0	50.0	56.2	59.1	0.0	0.0	2.890859	62434	63067	658-724	1.000000	66.8
rpl33-rps18_p4	rpl33-rps18	4	ACATACAATTCACGGGGAGA	AATTTGATCCCCCGATTGGA	20	20	45.0	45.0	56.2	56.9	0.0	0.0	2.903903	62434	63067	683-749	1.000000	66.5
rpl33-rps18_p5	rpl33-rps18	5	ACATACAATTCACGGGGAGA	CAATTTGATCCCCCGATTGG	20	20	45.0	50.0	56.2	56.8	0.0	0.0	2.944898	62434	63067	684-750	1.000000	66.1
rps12-clpP_p1	rps12-clpP	1	TGCACCAACGGAAACCATAA	AACGTATGGCATTCCCTCAC	20	20	45.0	50.0	58.0	58.0	0.0	4.8	0.055727	64881	65086	721-735	1.000000	85.0
rps12-clpP_p2	rps12-clpP	2	TTGCACCAACGGAAACCATA	AACGTATGGCATTCCCTCAC	20	20	45.0	50.0	58.0	58.0	0.0	4.8	0.055727	64881	65086	722-736	1.000000	85.0
rps12-clpP_p3	rps12-clpP	3	TGCACCAACGGAAACCATAA	CTGGGTGATAGCCTGGGATA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.047367	64881	65086	572-572	0.166667	46.3
rps12-clpP_p4	rps12-clpP	4	TTGCACCAACGGAAACCATA	CTGGGTGATAGCCTGGGATA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.047367	64881	65086	573-573	0.166667	46.3
rps12-clpP_p5	rps12-clpP	5	GATTGGCTGTCTTGCGTTTC	CTGGGTGATAGCCTGGGATA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.060924	64881	65086	342-342	0.166667	46.2
clpP_p1	clpP	1	TGCACCAACGGAAACCATAA	TGTTACGCTTCCACGTCAAA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.080062	65087	66503	1936-1952	1.000000	83.5
clpP_p2	clpP	2	GATTGGCTGTCTTGCGTTTC	TCCAAAAACCCGGATTCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.7	0.046831	65087	66503	2346-2456	1.000000	83.3
clpP_p3	clpP	3	TGCACCAACGGAAACCATAA	TCCAAAAACCCGGATTCGTT	20	20	45.0	45.0	58.0	58.0	0.0	0.7	0.033274	65087	66503	2576-2686	1.000000	83.2
clpP_p4	clpP	4	TTGCACCAACGGAAACCATA	TCCAAAAACCCGGATTCGTT	20	20	45.0	45.0	58.0	58.0	0.0	0.7	0.033274	65087	66503	2577-2687	1.000000	83.2
clpP_p5	clpP	5	TGCACCAACGGAAACCATAA	TTGTTACGCTTCCACGTCAA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.080062	65087	66503	1940-1953	0.666667	68.8
petB_p1	petB	1	GCCCAGTCTGTTTTGGTAGT	ACTATTGGCTGTTCCTCCCC	20	20	50.0	55.0	57.7	59.1	0.0	0.0	1.352234	69379	70836	1569-1589	1.000000	70.5
petB_p2	petB	2	GCCCAGTCTGTTTTGGTAGT	GCTGTTCCTCCCCCAAGTAA	20	20	50.0	55.0	57.7	59.3	0.0	0.0	1.575443	69379	70836	1562-1582	1.000000	68.7
petB_p3	petB	3	AGCCCAGTCTGTTTTGGTAG	ACTATTGGCTGTTCCTCCCC	20	20	50.0	55.0	57.4	59.1	0.0	0.0	1.648913	69379	70836	1570-1590	1.000000	68.1
petB_p4	petB	4	TGTTTTGGTAGTTCGACCGT	ACTATTGGCTGTTCCTCCCC	20	20	45.0	55.0	57.4	59.1	0.0	0.0	1.680760	69379	70836	1561-1581	1.000000	67.9
petB_p5	petB	5	GCCCAGTCTGTTTTGGTAGT	CTGTTCCTCCCCCAAGTAAT	20	20	50.0	50.0	57.7	56.5	0.0	0.0	1.772970	69379	70836	1561-1581	1.000000	67.1
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	ATAGCGCAGAACAGAATGGG	GAGCTCGGAGAAGGAAGAGA	20	20	50.0	55.0	58.0	58.2	11.5	9.7	0.283276	101541	102035	688-949	1.000000	84.4
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	TCGGGAGAATCGATGACTGA	GAGCTCGGAGAAGGAAGAGA	20	20	50.0	55.0	57.9	58.2	0.0	9.7	0.295100	101541	102035	834-1095	1.000000	83.9
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AGCTATGTAACTTGGGCGTG	GAGCTCGGAGAAGGAAGAGA	20	20	50.0	55.0	58.0	58.2	0.0	9.7	0.266493	101541	102035	1095-1346	1.000000	83.8
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TTTTGGTTTTACGCCCCGTA	GAGCTCGGAGAAGGAAGAGA	20	20	45.0	55.0	58.0	58.2	0.0	9.7	0.269446	101541	102035	972-1232	1.000000	83.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GGCAGAATAGCAGAGCAAGT	GAGCTCGGAGAAGGAAGAGA	20	20	50.0	55.0	58.0	58.2	0.0	9.7	0.277848	101541	102035	931-1191	1.000000	83.8
ycf1_p1	ycf1	1	TCAATCTATTATGCGCCGCT	CCAAAAGTTGCCAAACTGGG	20	20	45.0	50.0	57.5	58.0	11.7	0.0	0.503481	102969	103088	1097-1208	1.000000	81.0
ycf1_p2	ycf1	2	GCGTTCACGGTTTATTCCTG	CCAAAAGTTGCCAAACTGGG	20	20	50.0	50.0	57.5	58.0	0.8	0.0	0.568493	102969	103088	943-1054	1.000000	80.8
ycf1_p3	ycf1	3	CAATCTATTATGCGCCGCTG	CCAAAAGTTGCCAAACTGGG	20	20	50.0	50.0	57.5	58.0	11.7	0.0	0.563449	102969	103088	1096-1207	1.000000	80.6
ycf1_p4	ycf1	4	TCAATCTATTATGCGCCGCT	TGCCAAACTGGGAAGGTATG	20	20	45.0	50.0	57.5	57.8	11.7	0.0	0.670050	102969	103088	1089-1200	1.000000	79.7
ycf1_p5	ycf1	5	TCAATCTATTATGCGCCGCT	GCCAAACTGGGAAGGTATGT	20	20	45.0	50.0	57.5	57.8	11.7	0.0	0.671616	102969	103088	1088-1199	1.000000	79.7
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AAAAGGCCCGAAGTAGCAAA	AAGTGGACTGCGACAACAAT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042975	117400	118761	2383-2444	1.000000	83.2
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCAATTCCA	AAGTGGACTGCGACAACAAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.125988	117400	118761	1861-1902	1.000000	83.1
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCAATTCCAC	AAGTGGACTGCGACAACAAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.127444	117400	118761	1860-1901	1.000000	83.1
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	ACGACTGAAACGACCGAAAA	AAGTGGACTGCGACAACAAT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.107452	117400	118761	2092-2153	1.000000	83.0
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AAAAGGCCCGAAGTAGCAAA	CGGTAGATAAGTGGACTGCG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.131410	117400	118761	2391-2452	1.000000	82.5
rpl32-ndhF_p1	rpl32-ndhF	1	CAAAGCTTTCAACGCTGTCC	TTGGAAGTGGACTGAAAGGT	20	20	50.0	45.0	58.2	56.6	9.2	0.0	1.659895	118936	119882	1093-1118	1.000000	69.4
rpl32-ndhF_p2	rpl32-ndhF	2	CCCTTCTTCTTCCACACGTT	TTGGAAGTGGACTGAAAGGT	20	20	50.0	45.0	57.7	56.6	0.0	0.0	1.674796	118936	119882	1068-1093	1.000000	69.3
rpl32-ndhF_p3	rpl32-ndhF	3	TCAACGCTGTCCAATATCCC	TTGGAAGTGGACTGAAAGGT	20	20	50.0	45.0	57.7	56.6	0.0	0.0	1.744261	118936	119882	1085-1110	1.000000	68.8
rpl32-ndhF_p4	rpl32-ndhF	4	CAACGCTGTCCAATATCCCT	TTGGAAGTGGACTGAAAGGT	20	20	50.0	45.0	57.7	56.6	0.0	0.0	1.750742	118936	119882	1084-1109	1.000000	68.7
rpl32-ndhF_p5	rpl32-ndhF	5	AGCTTTCAACGCTGTCCAAT	TTGGAAGTGGACTGAAAGGT	20	20	45.0	45.0	58.4	56.6	0.0	0.0	1.809471	118936	119882	1090-1115	1.000000	68.2