Hotspot

rpoC2

LSC · 17711-21913 · 4203 bp

Back to Dipsacus

Pi 0.000952

MarkerSeek score 51.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4203
variable_sites: 6
pi: 0.000952
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCATTTCGAGGACTCACAG	GACCCCCAAGGACAAATGAT	55.0	50.0	58.0	57.5	237 bp	1.0	76.3
rpoC2_p2	CCCATTTCGAGGACTCACAG	GAGGATTAATGTCGGACCCC	55.0	55.0	58.0	57.5	251 bp	1.0	76.1
rpoC2_p3	TCACAGAAATACCTTGGGCG	GACCCCCAAGGACAAATGAT	50.0	50.0	57.9	57.5	223 bp	1.0	75.6
rpoC2_p4	TCACAGAAATACCTTGGGCG	GAGGATTAATGTCGGACCCC	50.0	55.0	57.9	57.5	237 bp	1.0	75.4
rpoC2_p5	CCCATTTCGAGGACTCACAG	GATTAATGTCGGACCCCCAA	55.0	50.0	58.0	57.3	248 bp	1.0	74.7