Hotspot

clpP

LSC · 73506-75608 · 2103 bp

Back to Dipsacus

Pi 0.00349

MarkerSeek score 45.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2103
variable_sites: 11
pi: 0.00349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TATTTCCGCGCAAGGATTCA	50.0	45.0	58.0	58.0	2840-2846 bp	1.0	80.2
clpP_p2	CTGAACGAGTCGCACATACA	TTTCCGCGCAAGGATTCATA	50.0	45.0	58.0	58.0	2838-2844 bp	1.0	80.2
clpP_p3	ATGATCTGAACGAGTCGCAC	TTTCCGCGCAAGGATTCATA	50.0	45.0	58.1	58.0	2843-2849 bp	1.0	79.7
clpP_p4	GATCTGAACGAGTCGCACAT	TATTTCCGCGCAAGGATTCA	50.0	45.0	58.1	58.0	2843-2849 bp	1.0	79.7
clpP_p5	GATCTGAACGAGTCGCACAT	TTTCCGCGCAAGGATTCATA	50.0	45.0	58.1	58.0	2841-2847 bp	1.0	79.7