Hotspot

cemA

LSC · 64196-64885 · 690 bp

Back to Dipsacus

Pi 0.00483

MarkerSeek score 55.10

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 5
pi: 0.00483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TTCATTGGCTCGATCCCTTG	GAATCTCCGGGATGGGTAGA	50.0	55.0	58.0	58.0	905 bp	1.0	80.0
cemA_p2	AGATCGCATAGAGTCGACGA	GAATCTCCGGGATGGGTAGA	50.0	55.0	58.1	58.0	856 bp	1.0	79.3
cemA_p3	CGAACGAGGTGGGTTCATTA	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.6	58.0	839 bp	1.0	77.4
cemA_p4	ATCGCATAGAGTCGACGAAC	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.6	58.0	854 bp	1.0	77.3
cemA_p5	AACGAGGTGGGTTCATTACC	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.5	58.0	837 bp	1.0	76.5