Hotspot

petA-psbJ

LSC · 66075-67072 · 998 bp

Back to Dipsacus

Pi 0.00134

MarkerSeek score 59.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGACTGGG	50.0	55.0	58.0	57.7	1150 bp	1.0	77.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGACTGGG	50.0	55.0	57.8	57.7	1133 bp	1.0	76.9
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGACTGGG	50.0	55.0	58.2	57.7	1147 bp	1.0	76.7
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	GGTTCATATTCCGGACTGGG	45.0	55.0	57.6	57.7	1145 bp	1.0	75.3
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	GGTTCATATTCCGGACTGGG	50.0	55.0	58.4	57.7	1144 bp	1.0	74.9