Hotspot

ycf4-cemA

LSC · 63252-64195 · 944 bp

Back to Dipsacus

Pi 0.00212

MarkerSeek score 56.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 3
pi: 0.00212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1072-1080 bp	1.0	78.4
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	CAGGGCAAAAAGACGAGAGA	45.0	50.0	57.8	57.8	1061-1069 bp	1.0	77.6
ycf4-cemA_p3	GCGGAATTGGCCTATTTCCT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.3	58.1	1062-1070 bp	1.0	77.2
ycf4-cemA_p4	GGCCATTCCTTTGACTCGTA	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.6	58.1	1123-1131 bp	1.0	76.4
ycf4-cemA_p5	GCGGAATTGGCCTATTTCCT	CAGGGCAAAAAGACGAGAGA	50.0	50.0	58.3	57.8	1051-1059 bp	1.0	76.4