Hotspot

rbcL-accD

LSC · 58473-59806 · 1334 bp

Back to Dipsacus

Pi 0.01

MarkerSeek score 65.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1334
variable_sites: 20
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	TGCTTGTATGAGGGAGTGGA	50.0	50.0	58.0	58.0	2164-2383 bp	1.0	84.2
rbcL-accD_p2	ATGAAATTATCCGTGCGGCT	TGCTTGTATGAGGGAGTGGA	45.0	50.0	58.0	58.0	2133-2352 bp	1.0	84.2
rbcL-accD_p3	AATGAGGGACGTGATCTTGC	GAGGATGAGTTCGGCGAATT	50.0	50.0	58.0	58.1	1679-1999 bp	1.0	80.4
rbcL-accD_p4	ATGAAATTATCCGTGCGGCT	GAGGATGAGTTCGGCGAATT	45.0	50.0	58.0	58.1	1648-1968 bp	1.0	80.4
rbcL-accD_p5	ATGAAATTATCCGTGCGGCT	CGGAAGCCTCCTCAATAGAAC	45.0	52.4	58.0	58.2	1466-1685 bp	1.0	71.6