Hotspot

rpoB-trnC-GCA

LSC · 28189-29490 · 1302 bp

Back to Dipsacus

Pi 0.00102

MarkerSeek score 55.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1302
variable_sites: 2
pi: 0.00102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATCAAGCCCTGCTCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1460-1461 bp	1.0	77.5
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1388-1389 bp	1.0	77.2
rpoB-trnC-GCA_p3	GTCCCTCATTTCCATCCTGG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.6	58.1	1389-1390 bp	1.0	76.6
rpoB-trnC-GCA_p4	TCAAGCCCTGCTCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1458-1459 bp	1.0	75.8
rpoB-trnC-GCA_p5	AAAGTTCGTCCATCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1470-1471 bp	1.0	75.7