primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	atpH-atpI	1	GCCAACTCCAGGTCCAATAG	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.0	58.5	0.0	0.0	0.561845	14678	15860	1320-1320	1.000000	75.7
atpH-atpI_p2	atpH-atpI	2	GCCAACTCCAGGTCCAATAG	CAAGCGGGATTCAAGCTCTT	20	20	55.0	50.0	58.0	58.5	0.0	5.7	0.586776	14678	15860	1341-1341	1.000000	75.5
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	14678	15860	1287-1287	1.000000	74.9
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.687386	14678	15860	1279-1279	1.000000	74.7
atpH-atpI_p5	atpH-atpI	5	AGCCAATCCAGCAGCAATAA	CAAGCGGGATTCAAGCTCTT	20	20	45.0	50.0	57.9	58.5	0.0	5.7	0.691099	14678	15860	1308-1308	1.000000	74.7
rps2_p1	rps2	1	CCCTTTTGCTAAGACCTGCA	GTACACCCTTCAAGGCAACA	20	20	50.0	50.0	58.1	58.0	1.4	0.0	0.114641	16814	17524	1785-1785	1.000000	79.3
rps2_p2	rps2	2	AGAAATTCTGACTGCCGGTC	GTACACCCTTCAAGGCAACA	20	20	50.0	50.0	57.9	58.0	1.9	0.0	0.124945	16814	17524	1530-1530	1.000000	79.3
rps2_p3	rps2	3	TTCTACGCCGGATATCTCGT	GTACACCCTTCAAGGCAACA	20	20	50.0	50.0	58.1	58.0	5.0	0.0	0.136126	16814	17524	1379-1379	1.000000	79.2
rps2_p4	rps2	4	GGACTTGGAAACCGCCTATT	GTACACCCTTCAAGGCAACA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148618	16814	17524	1422-1422	1.000000	79.1
rps2_p5	rps2	5	CCCTTTTGCTAAGACCTGCA	GATACGCCGGAACAATCAGT	20	20	50.0	50.0	58.1	58.1	1.4	5.0	0.154368	16814	17524	1653-1653	1.000000	79.0
rps2-rpoC2_p1	rps2-rpoC2	1	CCATGGCCAAAATGAACTCC	GATACGCCGGAACAATCAGT	20	20	50.0	50.0	57.3	58.1	11.0	5.0	0.742538	17525	17710	332-332	1.000000	75.3
rps2-rpoC2_p2	rps2-rpoC2	2	CCATGGCCAAAATGAACTCC	TACGATACGCCGGAACAATC	20	20	50.0	50.0	57.3	57.9	11.0	5.0	0.802482	17525	17710	335-335	1.000000	74.8
rps2-rpoC2_p3	rps2-rpoC2	3	GCCAAAATGAACTCCTGCCT	GATACGCCGGAACAATCAGT	20	20	50.0	50.0	58.7	58.1	0.0	5.0	0.803523	17525	17710	327-327	1.000000	74.8
rps2-rpoC2_p4	rps2-rpoC2	4	CCATGGCCAAAATGAACTCC	CTACGATACGCCGGAACAAT	20	20	50.0	50.0	57.3	57.9	11.0	5.0	0.808294	17525	17710	336-336	1.000000	74.7
rps2-rpoC2_p5	rps2-rpoC2	5	GCCAAAATGAACTCCTGCCT	TACGATACGCCGGAACAATC	20	20	50.0	50.0	58.7	57.9	0.0	5.0	0.863467	17525	17710	330-330	1.000000	74.3
rpoC2_p1	rpoC2	1	CCCATTTCGAGGACTCACAG	GACCCCCAAGGACAAATGAT	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.547985	21294	21413	237-237	1.000000	76.3
rpoC2_p2	rpoC2	2	CCCATTTCGAGGACTCACAG	GAGGATTAATGTCGGACCCC	20	20	55.0	55.0	58.0	57.5	0.0	5.6	0.561273	21294	21413	251-251	1.000000	76.1
rpoC2_p3	rpoC2	3	TCACAGAAATACCTTGGGCG	GACCCCCAAGGACAAATGAT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.640200	21294	21413	223-223	1.000000	75.6
rpoC2_p4	rpoC2	4	TCACAGAAATACCTTGGGCG	GAGGATTAATGTCGGACCCC	20	20	50.0	55.0	57.9	57.5	0.0	5.6	0.653487	21294	21413	237-237	1.000000	75.4
rpoC2_p5	rpoC2	5	CCCATTTCGAGGACTCACAG	GATTAATGTCGGACCCCCAA	20	20	55.0	50.0	58.0	57.3	0.0	5.6	0.737161	21294	21413	248-248	1.000000	74.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CATCAAGCCCTGCTCAATGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	5.3	0.0	0.323192	28189	29490	1460-1461	1.000000	77.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.367403	28189	29490	1388-1389	1.000000	77.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GTCCCTCATTTCCATCCTGG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.437137	28189	29490	1389-1390	1.000000	76.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCAAGCCCTGCTCAATGAAC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.544081	28189	29490	1458-1459	1.000000	75.8
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAAGTTCGTCCATCAAGCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.547399	28189	29490	1470-1471	1.000000	75.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAAAGGCGGTAGGGACTAG	GCTAGATCCCCTCTTTCCCT	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.332813	31507	32441	1094-1106	1.000000	78.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GAAAGGCGGTAGGGACTAGA	GCTAGATCCCCTCTTTCCCT	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.337917	31507	32441	1093-1105	1.000000	78.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAAGGCGGTAGGGACTAGAA	GCTAGATCCCCTCTTTCCCT	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.353635	31507	32441	1092-1104	1.000000	78.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAGTAGAAAGGCGGTAGGGA	GCTAGATCCCCTCTTTCCCT	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.353635	31507	32441	1098-1110	1.000000	78.1
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGCGGTAGGGACTAGAATG	GCTAGATCCCCTCTTTCCCT	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.404880	31507	32441	1090-1102	1.000000	77.7
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	4.2	0.137348	37948	38131	289-291	1.000000	80.0
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	37948	38131	260-262	1.000000	79.6
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	ATGCGGATATGGTCGAATGG	ATTAGTTTTCAGCGCGGAGT	20	20	50.0	45.0	57.9	57.9	0.0	10.8	0.193903	37948	38131	341-343	1.000000	79.4
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	ATTAGTTTTCAGCGCGGAGT	20	20	50.0	45.0	57.8	57.9	0.0	10.8	0.264595	37948	38131	312-314	1.000000	78.9
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.278053	37948	38131	287-289	1.000000	78.9
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	TGCTTGTATGAGGGAGTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.078629	58473	59806	2164-2383	1.000000	84.2
rbcL-accD_p2	rbcL-accD	2	ATGAAATTATCCGTGCGGCT	TGCTTGTATGAGGGAGTGGA	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.081472	58473	59806	2133-2352	1.000000	84.2
rbcL-accD_p3	rbcL-accD	3	AATGAGGGACGTGATCTTGC	GAGGATGAGTTCGGCGAATT	20	20	50.0	50.0	58.0	58.1	0.0	26.4	0.088513	58473	59806	1679-1999	1.000000	80.4
rbcL-accD_p4	rbcL-accD	4	ATGAAATTATCCGTGCGGCT	GAGGATGAGTTCGGCGAATT	20	20	45.0	50.0	58.0	58.1	5.6	26.4	0.091356	58473	59806	1648-1968	1.000000	80.4
rbcL-accD_p5	rbcL-accD	5	ATGAAATTATCCGTGCGGCT	CGGAAGCCTCCTCAATAGAAC	20	21	45.0	52.4	58.0	58.2	5.6	0.0	1.205030	58473	59806	1466-1685	1.000000	71.6
ycf4-cemA_p1	ycf4-cemA	1	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	63252	64195	1072-1080	1.000000	78.4
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCGGAATTGG	CAGGGCAAAAAGACGAGAGA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.323199	63252	64195	1061-1069	1.000000	77.6
ycf4-cemA_p3	ycf4-cemA	3	GCGGAATTGGCCTATTTCCT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.3	58.1	21.7	0.0	0.384234	63252	64195	1062-1070	1.000000	77.2
ycf4-cemA_p4	ycf4-cemA	4	GGCCATTCCTTTGACTCGTA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.473234	63252	64195	1123-1131	1.000000	76.4
ycf4-cemA_p5	ycf4-cemA	5	GCGGAATTGGCCTATTTCCT	CAGGGCAAAAAGACGAGAGA	20	20	50.0	50.0	58.3	57.8	21.7	0.0	0.477422	63252	64195	1051-1059	1.000000	76.4
cemA_p1	cemA	1	TTCATTGGCTCGATCCCTTG	GAATCTCCGGGATGGGTAGA	20	20	50.0	55.0	58.0	58.0	2.2	0.0	0.053808	64196	64885	905-905	1.000000	80.0
cemA_p2	cemA	2	AGATCGCATAGAGTCGACGA	GAATCTCCGGGATGGGTAGA	20	20	50.0	55.0	58.1	58.0	10.5	0.0	0.143956	64196	64885	856-856	1.000000	79.3
cemA_p3	cemA	3	CGAACGAGGTGGGTTCATTA	GAATCTCCGGGATGGGTAGA	20	20	50.0	55.0	57.6	58.0	5.6	0.0	0.384663	64196	64885	839-839	1.000000	77.4
cemA_p4	cemA	4	ATCGCATAGAGTCGACGAAC	GAATCTCCGGGATGGGTAGA	20	20	50.0	55.0	57.6	58.0	10.5	0.0	0.397624	64196	64885	854-854	1.000000	77.3
cemA_p5	cemA	5	AACGAGGTGGGTTCATTACC	GAATCTCCGGGATGGGTAGA	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.495864	64196	64885	837-837	1.000000	76.5
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGACTGGG	20	20	50.0	55.0	58.0	57.7	0.0	1.8	0.294504	66075	67072	1150-1150	1.000000	77.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGACTGGG	20	20	50.0	55.0	57.8	57.7	0.0	1.8	0.410971	66075	67072	1133-1133	1.000000	76.9
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGACTGGG	20	20	50.0	55.0	58.2	57.7	13.8	1.8	0.434901	66075	67072	1147-1147	1.000000	76.7
petA-psbJ_p4	petA-psbJ	4	TTACGTGTCCAAGGCCTTTT	GGTTCATATTCCGGACTGGG	20	20	45.0	55.0	57.6	57.7	17.6	1.8	0.607823	66075	67072	1145-1145	1.000000	75.3
petA-psbJ_p5	petA-psbJ	5	TACGTGTCCAAGGCCTTTTG	GGTTCATATTCCGGACTGGG	20	20	50.0	55.0	58.4	57.7	17.6	1.8	0.656094	66075	67072	1144-1144	1.000000	74.9
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	67863	68896	1216-1226	1.000000	73.0
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	67863	68896	1139-1149	1.000000	72.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	67863	68896	1225-1235	1.000000	72.1
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	67863	68896	1230-1240	1.000000	72.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	67863	68896	1224-1234	1.000000	71.9
clpP_p1	clpP	1	CTGAACGAGTCGCACATACA	TATTTCCGCGCAAGGATTCA	20	20	50.0	45.0	58.0	58.0	13.6	8.7	0.054070	73506	75608	2840-2846	1.000000	80.2
clpP_p2	clpP	2	CTGAACGAGTCGCACATACA	TTTCCGCGCAAGGATTCATA	20	20	50.0	45.0	58.0	58.0	13.6	8.7	0.054070	73506	75608	2838-2844	1.000000	80.2
clpP_p3	clpP	3	ATGATCTGAACGAGTCGCAC	TTTCCGCGCAAGGATTCATA	20	20	50.0	45.0	58.1	58.0	13.6	8.7	0.116625	73506	75608	2843-2849	1.000000	79.7
clpP_p4	clpP	4	GATCTGAACGAGTCGCACAT	TATTTCCGCGCAAGGATTCA	20	20	50.0	45.0	58.1	58.0	13.6	8.7	0.116625	73506	75608	2843-2849	1.000000	79.7
clpP_p5	clpP	5	GATCTGAACGAGTCGCACAT	TTTCCGCGCAAGGATTCATA	20	20	50.0	45.0	58.1	58.0	13.6	8.7	0.116625	73506	75608	2841-2847	1.000000	79.7
ycf1_p1	ycf1	1	AGCCATCCATCCATATCCCA	TAGGAACTGGGAGTGGAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.111663	113351	115306	2652-2709	1.000000	81.9
ycf1_p2	ycf1	2	ACGCGGTTAGGATCGATCTA	TAGGAACTGGGAGTGGAAGG	20	20	50.0	55.0	58.1	58.0	24.0	0.0	0.159164	113351	115306	2433-2490	1.000000	81.7
ycf1_p3	ycf1	3	TTTCACGTCGTGTACAGGAC	TAGGAACTGGGAGTGGAAGG	20	20	50.0	55.0	57.9	58.0	30.0	0.0	0.171390	113351	115306	2597-2654	1.000000	81.5
ycf1_p4	ycf1	4	AGCCATCCATCCATATCCCA	GGGAGTGGAAGGAAGGGTAT	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.169585	113351	115306	2644-2701	1.000000	81.4
ycf1_p5	ycf1	5	ACGCGGTTAGGATCGATCTA	AACCAAAATCCCCAACACGA	20	20	50.0	45.0	58.1	57.9	24.0	0.0	0.186514	113351	115306	2940-2997	1.000000	81.1
ccsA_p1	ccsA	1	GGCAGTCCGTCTTCTAAAAGA	GGTTCTCGCCAATTCGTACA	21	20	47.6	50.0	57.7	58.3	0.0	0.0	1.597347	118948	119913	1136-1136	1.000000	67.6
ccsA_p2	ccsA	2	GGCAGTCCGTCTTCTAAAAG	GGTTCTCGCCAATTCGTACA	20	20	50.0	50.0	56.2	58.3	0.0	0.0	2.112084	118948	119913	1136-1136	1.000000	63.5
ccsA_p3	ccsA	3	GGCAGTCCGTCTTCTAAAAGA	GGGTTCTCGCCAATTCGTAC	21	20	47.6	55.0	57.7	59.0	0.0	0.0	2.309946	118948	119913	1137-1137	1.000000	61.9
ccsA_p4	ccsA	4	GGCAGTCCGTCTTCTAAAAGA	TCTCGCCAATTCGTACAAAGT	21	21	47.6	42.9	57.7	58.0	0.0	0.0	2.349261	118948	119913	1133-1133	1.000000	61.6
ccsA_p5	ccsA	5	GGCAGTCCGTCTTCTAAAAGA	CACAGGGTTCTCGCCAATT	21	19	47.6	52.6	57.7	58.1	0.0	0.0	2.367594	118948	119913	1141-1141	1.000000	61.5
ccsA-ndhD_p1	ccsA-ndhD	1	GCTCTTCAATCCGAGTGGTT	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	57.9	58.0	0.0	8.2	0.105187	119914	120162	971-973	1.000000	80.2
ccsA-ndhD_p2	ccsA-ndhD	2	GCTCTTCAATCCGAGTGGTT	GGACCGCGAGAGTTATTTGT	20	20	50.0	50.0	57.9	58.0	0.0	8.2	0.106575	119914	120162	970-972	1.000000	80.2
ccsA-ndhD_p3	ccsA-ndhD	3	GCTCTTCAATCCGAGTGGTT	CGTTTTTGATTCTGGACCGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.136858	119914	120162	983-985	1.000000	80.0
ccsA-ndhD_p4	ccsA-ndhD	4	GCTCTTCAATCCGAGTGGTT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.136858	119914	120162	982-984	1.000000	80.0
ccsA-ndhD_p5	ccsA-ndhD	5	GCTCTTCAATCCGAGTGGTT	TACAGGACTCAATGGAGCCA	20	20	50.0	50.0	57.9	58.0	0.0	11.7	0.149395	119914	120162	1367-1369	1.000000	79.6
rps15-ycf1_p1	rps15-ycf1	1	TCAGCAGATCCCCTGTTTTC	GGCAGGAATCGTTTGAGGAT	20	20	50.0	50.0	57.5	58.0	9.3	0.0	0.541021	128040	128438	553-553	1.000000	76.0
rps15-ycf1_p2	rps15-ycf1	2	CAGCAGATCCCCTGTTTTCT	GGCAGGAATCGTTTGAGGAT	20	20	50.0	50.0	57.5	58.0	2.8	0.0	0.548018	128040	128438	552-552	1.000000	75.9
rps15-ycf1_p3	rps15-ycf1	3	TCAGCAGATCCCCTGTTTTC	CACCATTAGAACGGCAGGAA	20	20	50.0	50.0	57.5	57.9	9.3	0.0	0.604848	128040	128438	565-565	1.000000	75.4
rps15-ycf1_p4	rps15-ycf1	4	CAGCAGATCCCCTGTTTTCT	CACCATTAGAACGGCAGGAA	20	20	50.0	50.0	57.5	57.9	2.8	0.0	0.611845	128040	128438	564-564	1.000000	75.4
rps15-ycf1_p5	rps15-ycf1	5	TCAGCAGATCCCCTGTTTTC	ATCCACCATTAGAACGGCAG	20	20	50.0	50.0	57.5	57.7	9.3	0.0	0.828962	128040	128438	568-568	1.000000	73.6