Hotspot

trnN-GUU-rpl32

SSC · 106211-107246 · 1036 bp

Back to Dipodium

Pi 0.0499
MarkerSeek score 89.90
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
1036
variable_sites
46
pi
0.0499
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG AGCTAAAGAAAAAGCTTTTACTACCT 50.0 30.8 58.1 57.3 1094-1184 bp 1.0 47.2
trnN-GUU-rpl32_p2 AAAGCGAGCCCTTTCATTCA AGCTAAAGAAAAAGCTTTTACTACCT 45.0 30.8 58.1 57.3 1169-1259 bp 1.0 47.0
trnN-GUU-rpl32_p3 AAGCGAGCCCTTTCATTCAA AGCTAAAGAAAAAGCTTTTACTACCT 45.0 30.8 58.1 57.3 1168-1258 bp 1.0 47.0
trnN-GUU-rpl32_p4 TCAAAGCGAGCCCTTTCATT AGCTAAAGAAAAAGCTTTTACTACCT 45.0 30.8 58.1 57.3 1171-1261 bp 1.0 47.0
trnN-GUU-rpl32_p5 AGCGAGCCCTTTCATTCAAT AGCTAAAGAAAAAGCTTTTACTACCT 45.0 30.8 57.9 57.3 1167-1257 bp 1.0 47.0