Hotspot

petD-rpoA

LSC · 75960-76102 · 143 bp

Back to Dipodium

Pi 0.0559

MarkerSeek score 67.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 143
variable_sites: 8
pi: 0.0559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTATGGAGAACCTGCATGGC	AAGAGAGGTCGCGGATATCA	50.0	50.0	57.9	58.0	1113 bp	1.0	80.3
petD-rpoA_p2	TTATGGAGAACCTGCATGGC	ATCAAGAGAGGTCGCGGATA	50.0	50.0	57.9	58.0	1116 bp	1.0	80.3
petD-rpoA_p3	TTATGGAGAACCTGCATGGC	TCAAGAGAGGTCGCGGATAT	50.0	50.0	57.9	58.0	1115 bp	1.0	80.3
petD-rpoA_p4	TCATGTCCGGTTCTTTTGGG	AAGAGAGGTCGCGGATATCA	50.0	50.0	58.1	58.0	1234 bp	1.0	79.9
petD-rpoA_p5	TCATGTCCGGTTCTTTTGGG	TCAAGAGAGGTCGCGGATAT	50.0	50.0	58.1	58.0	1236 bp	1.0	79.9