Hotspot

psbF-psbE

LSC · 62124-62133 · 10 bp

Back to Dipodium

Pi 0.1

MarkerSeek score 77.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 10
variable_sites: 1
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GCCAACCATCGCACTGTAAA	GAAGTCCTCGGCCAAATGAA	50.0	50.0	59.1	58.2	167 bp	1.0	70.1
psbF-psbE_p2	GCCAACCATCGCACTGTAAA	ATTTCACAGAGAGCCGACAG	50.0	50.0	59.1	57.6	146 bp	1.0	68.6
psbF-psbE_p3	CCAACCATCGCACTGTAAAA	GAAGTCCTCGGCCAAATGAA	45.0	50.0	56.6	58.2	166 bp	1.0	68.2
psbF-psbE_p4	GCCAACCATCGCACTGTAA	GAAGTCCTCGGCCAAATGAA	52.6	50.0	58.4	58.2	167 bp	1.0	67.5
psbF-psbE_p5	GCCAACCATCGCACTGTAAA	AAGTCCTCGGCCAAATGAAT	50.0	45.0	59.1	57.5	166 bp	1.0	67.4