Hotspot

accD-psaI

LSC · 56102-56836 · 735 bp

Back to Dipodium

Pi 0.0204

MarkerSeek score 58.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 15
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AGGAACAAAAATAGAGGGAAAGT	50.0	34.8	58.0	55.7	801-806 bp	1.0	41.5
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	GGAACAAAAATAGAGGGAAAGTTCA	50.0	36.0	58.0	57.7	800-805 bp	1.0	41.5
accD-psaI_p3	CACGGTTTCCTTCCCTTGAA	AGGAACAAAAATAGAGGGAAAGTTC	50.0	36.0	58.0	57.5	801-806 bp	1.0	41.5
accD-psaI_p4	CACGGTTTCCTTCCCTTGAA	AAGGAACAAAAATAGAGGGAAAGT	50.0	33.3	58.0	56.4	802-807 bp	1.0	41.5
accD-psaI_p5	CACGGTTTCCTTCCCTTGAA	AGGAACAAAAATAGAGGGAAAGTT	50.0	33.3	58.0	56.4	801-806 bp	1.0	41.5