Hotspot

rps16-trnQ-UUG

LSC · 5835-6733 · 899 bp

Back to Dipodium

Pi 0.01
MarkerSeek score 64.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
899
variable_sites
9
pi
0.01
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AATGGTGGATGCCAGAATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1011-1040 bp 1.0 79.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 972-1001 bp 1.0 79.0
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 983-1012 bp 1.0 78.5
rps16-trnQ-UUG_p4 AATGGTGGATGCCAGAATCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1015-1044 bp 1.0 78.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 976-1005 bp 1.0 77.9