Hotspot

ndhF

SSC · 124831-127056 · 2226 bp

Back to Diplostephium

Pi 0.00407

MarkerSeek score 41.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 45
pi: 0.00407
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAAAGGTAAGATCCGGTGA	TTCTTCCTTCTCCGAGCTCA	50.0	50.0	57.0	58.1	2736-2798 bp	0.851852	68.5
ndhF_p2	TCGAAAGGTAAGATCCGGTG	TTCTTCCTTCTCCGAGCTCA	50.0	50.0	57.0	58.1	2737-2799 bp	0.851852	68.5
ndhF_p3	CGAAAGGTAAGATCCGGTGA	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.0	57.9	2607-2669 bp	0.851852	68.1
ndhF_p4	TCGAAAGGTAAGATCCGGTG	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.0	57.9	2608-2670 bp	0.851852	68.1
ndhF_p5	CGAAAGGTAAGATCCGGTGA	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.0	58.2	2795-2857 bp	0.851852	67.7