Hotspot

ndhD

SSC · 119989-121491 · 1503 bp

Back to Diplostephium

Pi 0.00282

MarkerSeek score 40.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 25
pi: 0.00282
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTTTGGTCCAAGCGTATCTT	TCATTATCCAACCGACTCTT	40.0	40.0	55.3	53.8	1593 bp	1.0	41.4
ndhD_p2	TTTTGGTCCAAGCGTATCTT	TTCATTATCCAACCGACTCT	40.0	40.0	55.3	53.8	1594 bp	1.0	41.4
ndhD_p3	TTTTTGGTCCAAGCGTATCT	TCATTATCCAACCGACTCTT	40.0	40.0	55.3	53.8	1594 bp	1.0	41.4
ndhD_p4	TTTTTGGTCCAAGCGTATCT	TTCATTATCCAACCGACTCT	40.0	40.0	55.3	53.8	1595 bp	1.0	41.4
ndhD_p5	TTTTGGTCCAAGCGTATCTT	TCCAACCGACTCTTAAAAATCA	40.0	36.4	55.3	55.7	1587 bp	0.962963	39.7