Hotspot

ycf1-rps15

SSC · 113460-113862 · 403 bp

Back to Diplostephium

Pi 0.00976

MarkerSeek score 46.00

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 16
pi: 0.00976
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GGGAGCCGTTTGAGTATGTT	AGTCTTCGTATCTTGTTGGTGA	50.0	40.9	57.9	57.1	557-573 bp	0.962963	57.4
ycf1-rps15_p2	ATGGGAGCCGTTTGAGTATG	AGTCTTCGTATCTTGTTGGTGA	50.0	40.9	57.7	57.1	559-575 bp	0.962963	55.6
ycf1-rps15_p3	GGGAGCCGTTTGAGTATGTT	AAGTCTTCGTATCTTGTTGGTGA	50.0	39.1	57.9	57.8	558-574 bp	0.962963	54.7
ycf1-rps15_p4	GGGAGCCGTTTGAGTATGTT	AGTCTTCGTATCTTGTTGGTGAA	50.0	39.1	57.9	57.8	557-573 bp	0.962963	54.7
ycf1-rps15_p5	GGGAGCCGTTTGAGTATGTT	GTCTTCGTATCTTGTTGGTGA	50.0	42.9	57.9	55.7	556-572 bp	0.962963	53.7