Hotspot

petA-psbJ

LSC · 62981-63694 · 714 bp

Back to Diplostephium

Pi 0.00621

MarkerSeek score 56.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 21
pi: 0.00621
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	CCGGATTGGGTTCATCTCTG	45.0	55.0	58.0	58.0	848-849 bp	1.0	81.5
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.9	58.0	838-839 bp	1.0	81.5
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TCCGGATTGGGTTCATCTCT	45.0	50.0	58.0	58.1	849-850 bp	1.0	80.8
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.9	58.1	839-840 bp	1.0	80.8
petA-psbJ_p5	TCCATTACGTGTACAAGGCC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.6	58.0	854-855 bp	1.0	78.7