Hotspot

ycf4-cemA

LSC · 60218-61090 · 873 bp

Back to Diplostephium

Pi 0.00616

MarkerSeek score 57.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 22
pi: 0.00616
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	496-1079 bp	1.0	85.0
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	460-1043 bp	1.0	84.6
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	495-1078 bp	1.0	82.5
ycf4-cemA_p4	CTCCACGGGAAATGGAACAA	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.1	57.6	459-1042 bp	1.0	82.0
ycf4-cemA_p5	GGAGCCATTCCATTGACTCG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.7	58.1	497-1080 bp	1.0	79.7