Hotspot

accD-psaI

LSC · 58297-59182 · 886 bp

Back to Diplostephium

Pi 0.0127

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 57
pi: 0.0127
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCCAGTTTTGGTA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	793-1242 bp	1.0	87.9
accD-psaI_p2	GGTGACAGCCAGTTTTGGTA	GATTTCGACGGAAGCCTCTT	50.0	50.0	58.0	58.0	937-1384 bp	1.0	87.8
accD-psaI_p3	TACTACTGGTGGGGTGACAG	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.1	58.0	805-1254 bp	1.0	87.6
accD-psaI_p4	GACAGGATTAACCGAGGCTG	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.1	58.0	1108-1557 bp	1.0	86.8
accD-psaI_p5	GGTGACAGCCAGTTTTGGTA	ATAAAAAGACCCGCGATCCC	50.0	50.0	58.0	58.0	1426-1873 bp	1.0	86.8