Hotspot

ndhC-trnV-UAC

LSC · 50435-51145 · 711 bp

Back to Diplostephium

Pi 0.00954
MarkerSeek score 73.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
711
variable_sites
40
pi
0.00954
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 841-884 bp 1.0 83.6
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 843-886 bp 1.0 82.7
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 846-889 bp 1.0 82.7
ndhC-trnV-UAC_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 840-883 bp 1.0 80.9
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 842-885 bp 1.0 80.5