Hotspot

trnT-GGU-psbD

LSC · 31292-32624 · 1333 bp

Back to Diplostephium

Pi 0.00802

MarkerSeek score 64.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 54
pi: 0.00802
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGACCCGTTCAGGAATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1503-1548 bp	0.888889	80.8
trnT-GGU-psbD_p2	CAGTGACCCGTTCAGGAATT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1505-1550 bp	0.888889	80.8
trnT-GGU-psbD_p3	GTAAGCCCTCGGTTCAGATC	GAAACGGTCCCTACGTAACC	55.0	55.0	57.8	58.0	1429-1474 bp	0.888889	80.5
trnT-GGU-psbD_p4	GTGACCCGTTCAGGAATTCA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1521-1566 bp	0.888889	79.9
trnT-GGU-psbD_p5	TACAGTGACCCGTTCAGGAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1507-1552 bp	0.888889	79.9