Hotspot

atpA-trnR-UCU

LSC · 29561-29695 · 135 bp

Back to Diplostephium

Pi 0.0283

MarkerSeek score 69.70

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 12
pi: 0.0283
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.8	58.3	234-249 bp	1.0	75.3
atpA-trnR-UCU_p2	TTTACCGAGGAAGCAGAAGC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	295-310 bp	1.0	74.6
atpA-trnR-UCU_p3	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.8	56.6	234-249 bp	1.0	74.5
atpA-trnR-UCU_p4	CAGGAACAGAGGGAACGTTT	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.7	58.3	256-271 bp	1.0	74.1
atpA-trnR-UCU_p5	TTTACCGAGGAAGCAGAAGC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	295-310 bp	1.0	73.7