Hotspot

rpoC2-rps2

LSC · 23008-23237 · 230 bp

Back to Diplostephium

Pi 0.011

MarkerSeek score 57.30

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 13
pi: 0.011
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TGCCTTTCAAAGAATTTCCATGA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.2	57.3	343-350 bp	1.0	48.5
rpoC2-rps2_p2	TGCCTTTCAAAGAATTTCCATGA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.2	58.7	339-346 bp	1.0	48.1
rpoC2-rps2_p3	TCTTGCCTTTCAAAGAATTTCCA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.1	57.3	346-353 bp	1.0	48.0
rpoC2-rps2_p4	TCTTGCCTTTCAAAGAATTTCCA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.1	58.7	342-349 bp	1.0	47.6
rpoC2-rps2_p5	TGCCTTTCAAAGAATTTCCATGA	TGGCCAAAATGAACTCCTGC	34.8	50.0	57.2	59.0	340-347 bp	1.0	45.7