Hotspot

trnY-GUA-rpoB

LSC · 11515-12736 · 1222 bp

Back to Diplostephium

Pi 0.00635

MarkerSeek score 60.30

Primer available yes

Label trnY-GUA-rpoB

Feature Metadata

label_name: trnY-GUA-rpoB
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 40
pi: 0.00635
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-rpoB_p1	CAACGAATTTACAGTCCGCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	57.5	56.8	1336-1387 bp	0.962963	62.5
trnY-GUA-rpoB_p2	AACGAATTTACAGTCCGCCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.6	56.8	1335-1386 bp	0.962963	62.3
trnY-GUA-rpoB_p3	AGATTGGCAAATTGTTGGGC	TCAAACCCTGATCAATGAACCT	45.0	40.9	57.5	57.6	1379-1430 bp	0.962963	61.4
trnY-GUA-rpoB_p4	AGATTGGCAAATTGTTGGGC	AGTTCTTCTGTCAAACCCTGA	45.0	42.9	57.5	56.8	1389-1440 bp	0.925926	60.8
trnY-GUA-rpoB_p5	GATTGGCAAATTGTTGGGCC	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.5	56.8	1388-1439 bp	0.925926	60.2