Hotspot

psbI-trnS-GCU

LSC · 8278-8424 · 147 bp

Back to Diplostephium

Pi 0.0186

MarkerSeek score 64.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 13
pi: 0.0186
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	257-283 bp	1.0	77.7
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	205-231 bp	1.0	76.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	257-283 bp	1.0	75.4
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	276-302 bp	1.0	74.2
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	248-274 bp	1.0	73.7