Hotspot

rrn16-ndhB

Genome · 145129-149291 · 4163 bp

Back to Diplopterygium

Pi 0.0179

MarkerSeek score 64.80

Primer available yes

Label rrn16-ndhB

Feature Metadata

label_name: rrn16-ndhB
feature_type: igs
region: Genome
length_bp: 4163
variable_sites: 74
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-ndhB_p1	GCTGCTACACGTCAATACCA	GTTAGGCGGGATGTTCTGAG	50.0	55.0	58.0	58.1	1201-1318 bp	1.0	85.4
rrn16-ndhB_p2	TGCTGCTACACGTCAATACC	GTTAGGCGGGATGTTCTGAG	50.0	55.0	58.0	58.1	1202-1319 bp	1.0	85.4
rrn16-ndhB_p3	GCTGCTACACGTCAATACCA	ATTGAACTAGGGCTGGGACT	50.0	50.0	58.0	58.0	1411-1555 bp	1.0	84.8
rrn16-ndhB_p4	TGCTGCTACACGTCAATACC	ATTGAACTAGGGCTGGGACT	50.0	50.0	58.0	58.0	1412-1556 bp	1.0	84.8
rrn16-ndhB_p5	GCTGCTACACGTCAATACCA	TTAGGCGGGATGTTCTGAGA	50.0	50.0	58.0	58.1	1200-1317 bp	1.0	84.8