Hotspot

ycf1-chlN

Genome · 133690-133911 · 222 bp

Back to Diplopterygium

Pi 0.0138

MarkerSeek score 62.10

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 222
variable_sites: 3
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	TACAACGACATTCGAACGCT	TGGATTTACGAACGCGAGAG	45.0	50.0	57.9	58.1	365-369 bp	1.0	80.3
ycf1-chlN_p2	TACAACGACATTCGAACGCT	TTACGAACGCGAGAGATGTG	45.0	50.0	57.9	58.1	360-364 bp	1.0	80.2
ycf1-chlN_p3	AATAGTAGGGGAGGACCTGC	TGGATTTACGAACGCGAGAG	55.0	50.0	57.9	58.1	422-426 bp	1.0	79.7
ycf1-chlN_p4	AATAGTAGGGGAGGACCTGC	TTACGAACGCGAGAGATGTG	55.0	50.0	57.9	58.1	417-421 bp	1.0	79.6
ycf1-chlN_p5	TACAACGACATTCGAACGCT	GGATTTACGAACGCGAGAGA	45.0	50.0	57.9	57.8	364-368 bp	1.0	79.6