Hotspot

rpl32-trnP-GGG

Genome · 117430-118558 · 1129 bp

Back to Diplopterygium

Pi 0.0071

MarkerSeek score 61.60

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 1129
variable_sites: 8
pi: 0.0071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	TCTTCTTTAGCCCGCTCAAC	TAACGAACGGGATATAGCGC	50.0	50.0	57.9	57.7	1297-1303 bp	1.0	77.2
rpl32-trnP-GGG_p2	CTTCTTTAGCCCGCTCAACA	TAACGAACGGGATATAGCGC	50.0	50.0	58.2	57.7	1296-1302 bp	1.0	76.4
rpl32-trnP-GGG_p3	TTTAGCCCGCTCAACATTGA	TAACGAACGGGATATAGCGC	45.0	50.0	57.8	57.7	1292-1298 bp	1.0	76.4
rpl32-trnP-GGG_p4	CTTCTTCTTTAGCCCGCTCA	TAACGAACGGGATATAGCGC	50.0	50.0	57.6	57.7	1299-1305 bp	1.0	74.8
rpl32-trnP-GGG_p5	CCGGTCGTTCCAGAAGTTTT	TAACGAACGGGATATAGCGC	50.0	50.0	58.4	57.7	1272-1278 bp	1.0	74.7