Hotspot

petB-petD

Genome · 89830-90096 · 267 bp

Back to Diplopterygium

Pi 0.03

MarkerSeek score 60.10

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: Genome
length_bp: 267
variable_sites: 8
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	GCTCCTTACGGCCGTTTTTA	GCGGTATTCGATGGGGTATC	50.0	55.0	58.6	58.0	405-409 bp	1.0	77.1
petB-petD_p2	GCTCCTTACGGCCGTTTTTA	TCGATGGGGTATCTGCATCT	50.0	50.0	58.6	58.0	398-402 bp	1.0	77.0
petB-petD_p3	GCTCCTTACGGCCGTTTTTA	CGATGGGGTATCTGCATCTG	50.0	55.0	58.6	57.9	397-401 bp	1.0	76.4
petB-petD_p4	GCTCCTTACGGCCGTTTTTA	GCATCTGCATAGCCTACGTT	50.0	50.0	58.6	58.1	384-388 bp	1.0	76.2
petB-petD_p5	GCTCCTTACGGCCGTTTTTA	AGATGTGGCGGTATTCGATG	50.0	50.0	58.6	57.8	412-416 bp	1.0	75.8