Hotspot

rps12-clpP

Genome · 83074-83597 · 524 bp

Back to Diplopterygium

Pi 0.00771

MarkerSeek score 62.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 524
variable_sites: 4
pi: 0.00771
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTTCATCGGTTGTCTTGCCT	GGGGGAATGTATCACGGAAG	50.0	55.0	58.2	57.7	637-642 bp	1.0	77.6
rps12-clpP_p2	CTTCATCGGTTGTCTTGCCT	GGGAATGTATCACGGAAGCA	50.0	50.0	58.2	57.7	635-640 bp	1.0	77.0
rps12-clpP_p3	TCATCGGTTGTCTTGCCTTT	GGGGGAATGTATCACGGAAG	45.0	55.0	57.7	57.7	635-640 bp	1.0	76.9
rps12-clpP_p4	TTCATCGGTTGTCTTGCCTT	GGGGGAATGTATCACGGAAG	45.0	55.0	57.7	57.7	636-641 bp	1.0	76.9
rps12-clpP_p5	CTTCATCGGTTGTCTTGCCT	GGGGAATGTATCACGGAAGC	50.0	55.0	58.2	58.4	636-641 bp	1.0	76.4